Large-scale proteome and phosphoproteome analyses of FLA2 and FLB1 leukemias. (A) Results of protein expression experiments. (B) Results of phosphoproteome experiments. (C-D) Volcano plot of the protein abundance data: levels of 218 and 224 proteins change significantly in nuclear and cytosolic extracts, respectively (≥ 2-fold change in abundance). (E) Phosphosite confidence level. A total of 6622 phosphosites were identified with a high confidence level (class I), whereas 2080 and 757 sites were assigned with medium and low confidence, respectively. (F) Distribution of phosphorylation sites by amino acids showing a relatively large proportion of phosphorylation at serine residues. (G-H) Volcano plot of the phosphopeptide experiments: 262 and 1054 phosphopeptides change significantly in nuclear and cytosolic extracts, respectively (≥ 2-fold change in abundance).