IFNγ⁺ T-cell responses to equimolar mixtures of 9-mer peptides identified by epitope mapping of in vitro responses and peptides within the same 15-mer or adjacent overlapping 15-mer peptides predicted to have higher binding affinity and immunogenicity. (A) Responses to a mixture of nonamers spanning amino acids +2 to +31 including the _6-15RDL and _22-31GGC peptides to which HLA A0201⁺ donors responded in epitope-mapping studies. (B) Responses to the in vitro–mapped _{(−75)-(−67)}AILDFLLLQ epitope and a flanking peptide _{(−78)-(−70)}LLAAILDFL with higher predicted binding affinity. (C) Responses to the in vitro–mapped _38-46LDFAPPGAS epitope and the overlapping _37-45VLDFAPPGA predicted to have higher binding affinity.