Schema of WT-1. (A) Schema of WT-1 DNA. (B) Schema of WT-1 mRNA (includes region encoding first 126 amino acids within the N-terminus reported by Gessler et al³⁷ ). (C) Localization of the previously described immunogenic epitopes presented through HLA class I (dark gray) and HLA class II (light gray) either predicted (p) by the computer algorithm or identified (i) by the comparative analysis of the immunogenicity of a panel of neighboring peptides. The 126 amino acids at the N-terminus are numbered with negative values from the first AUG codon in exon 1. (D) Localization of the immunogenic epitopes identified in this study by the epitope-mapping approach presented by HLA class I (yellow) and HLA class II (pink) alleles and marked within the schema of the WT-1 protein 575 amino acids in length, including 126 amino acids labeled with negative numbers at the N-terminus upstream from the first AUG codon initiating exon 1. (E) Number of healthy donors responding to the identified epitopes (yellow) or cluster of epitopes (gray). (F) Distribution of the functional activities among the corresponding regions of the WT-1 protein.