Comparison of prophylactic treatment with the different FIX agents in CRM⁺ mice. (A) SVBA 7 days after the infusion of 150 IU/kg FIX_WT, FIX_FC, or FIX_Alb in CRM⁺ mice. Results for untreated CRM⁺ and wild-type mice are also shown. (B) SVBA 7 days after the infusion of extremely high doses of FIX_WT, FIX_FC, and FIX_Alb in CRM⁺ mice. Untreated CRM⁺ mice (orange), FIX_WT (green), FIX_FC (purple), FIX_Alb (blue), WT mice (red). Box plot rendition of the results showing the minimum, first quartile, median (waist), third quartile, and maximum values. Note no difference between agents at the 150 IU/kg dose, but significantly increased efficacy with FIX_FC at very high doses.