Time course of hemostasis after infusions of FIX_WT and FIX_FC in CRM⁻ and CRM⁺ mice. (A) SVBA at 1 and 7 days after the infusion of 150 IU/kg FIX_WT (green) or FIX_FC (purple) in CRM⁻ mice. (B) SVBA at 1, 3, 5, and 7 days after the infusion of 150 IU/kg FIX_WT (green) or FIX_FC (purple) in CRM⁺ mice. (C) Plot of median SVBA values for FIX_WT (red) and FIX_FC (blue) in CRM⁻ mice and for FIX_WT (green) and FIX_FC (orange) in CRM⁺ mice from panels A and B after adjustment for the median SVBA baseline values of untreated CRM⁻ (1) and CRM⁺ (3) mice. Note no significant difference in the hemostatic effects of the agents in CRM⁺ or CRM⁻ mice at 24 hours, but the rate of decay in hemostatic effectiveness is much faster in CRM⁺ compared with CRM⁻ mice. At 7 days, the differences between SVBA results in CRM⁻ and CRM⁺ mice are statistically significant for both FIX_WT (P=.001) and FIX_FC (P = .02) treatments.