Times to first clot for the different FIX agents in CRM⁻ mice. The saphenous vein of CRM⁻ mice was injured and the mice were allowed to bleed for 15 minutes. Each FIX agent (500 IU/kg) was then infused and the TTFC (see Methods) determined. FIX_WT (green), FIX_FC (purple) and FIX_Alb (blue). FIX_WTAct and FIX_FCAct are the activated forms of the respective zymogens. Box plot rendition of the results showing the minimum, first quartile, median (waist), third quartile, and maximum values.