Flow cytometric detection of MRD after induction chemotherapy in the BM of an AML patient led by the patient's LAIP. In the Scattergram, a gate (P1) is set around cells with low forward scatter (FSC) and side scatter (SSC), reflecting small to intermediate size and low granularity, respectively. In the blast gate, cells (green dots) within P1 with high CD34 and intermediate CD45 expression are selected away (P2) from normal lymphocytes (red dots with high CD45 expression) and erythroid precursor cells (red dots lacking CD45). Gating based on the typically reduced intensity of staining with the pan-leukocyte marker CD45 facilitates the detection of leukemic cells. In the blast cell characterization contour plot, MRD is detected within the CD34^HIGHCD45^WEAK gate based on the dual expression of CD33 and CD7 (P3, blue cluster). These cells with the patient's LAIP features account for 0.013% of all nucleated cells, a common denominator for MRD definition. The remaining green cells in this plot represent normal myeloid precursor cells (CD33⁺CD7⁻) caught in P2.