Posttreatment ATLL cells in patients 1 and 3 represent distinct Treg subtypes. RNAseq was used to profile gene-expression changes caused by nivolumab treatment. The color bar indicates the fold change (after/before) for each gene. Selected genes represent genes associated with peripheral blood cell signaling pathways that increase or decrease more than twofold after treatment with nivolumab and differ between patient 1 and patient 3.