Temporal distribution of LM-SPM in the thrombus during DVT in mice. (A) Naturally occurring VT progression in C57BL/6J WT mice. Hematoxylin and eosin stains of longitudinal mouse venous thrombus sections at days 2, 4, 8, 12, 16, and 21 postinduction. The stenosis site (head of the thrombus) is to the left and the distal end of the thrombus (tail) is to the right on all images. Scale bars, 1 mm. (B) Representative liquid chromatography-tandem mass spectrometry chromatograms of the LMs derived from DHA (blue), from EPA (dark blue), and from AA (red) identified in the thrombus. (C) Tandem mass spectrometry spectra from SPMs identified in the thrombus by characteristic diagnostic ions. (D) Representative principal component analysis score plot (left) and loading plot (right) showing temporal distribution of LMs in the thrombus during DVT. Orange, early (acute) phase (6 hours, day 2, and day 4); purple, middle (subacute) phase (day 6 and day 8); green, late (chronic) phase (day 14) after induction of DVT in WT mice. Data in panels B, C, and D are representative of a pool of 3 thrombi.