Figure 3.
Neutrophil migration in a mouse model of pneumonia is L-selectin–dependent. (A-D) WT and Sell-/- mice were injected intratracheally with Kpneumoniae. (A-B) Representative confocal images and neutrophil migratory tracks in agarose-filled lung slices of wildtype (A) and Sell−/− (B) mice over a 60 minute timeframe 4 hours after intratracheal instillation of Kpneumoniae. The CellObserver SD (Zeiss, Göttingen, Germany) equipped with a 20 ×/1.0 NA objective was used, white scale bars represent 30 µm. Neutrophil migration velocity (C) and distance (D). n = at least 3 mice/genotype, mean ± standard error of the mean, rank-sum test *P = .05.

Neutrophil migration in a mouse model of pneumonia is L-selectin–dependent. (A-D) WT and Sell-/- mice were injected intratracheally with Kpneumoniae. (A-B) Representative confocal images and neutrophil migratory tracks in agarose-filled lung slices of wildtype (A) and Sell−/− (B) mice over a 60 minute timeframe 4 hours after intratracheal instillation of Kpneumoniae. The CellObserver SD (Zeiss, Göttingen, Germany) equipped with a 20 ×/1.0 NA objective was used, white scale bars represent 30 µm. Neutrophil migration velocity (C) and distance (D). n = at least 3 mice/genotype, mean ± standard error of the mean, rank-sum test *P = .05.

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