L-selectin regulates integrin outside-in signaling in neutrophils. Phagocytosis of pHrodo E coli (A) or K pneumoniae (B) particles by WT and Sell^−/− neutrophils that were left untreated or were pretreated with Mac-1– or LFA-1–blocking antibody. Adhesion-dependent oxidative burst of WT and Sell^−/− neutrophils plated on pRGD (C), ICAM-1 (D), or fibrinogen (E), alone or in the presence of TNF-α. WT and Sell^−/− neutrophils were plated on pRGD for 10 minutes, and lysates were immunoblotted with anti–phosphorylated (p)-PLCγ2 or anti-PLCγ2 (F-G) or anti-phosphotyrosine (4G10) or total Syk (tSyk) (F,H) after immunoprecipitation for Syk or immunoprecipitation using an IgG control antibody and anti–p-Akt and anti-Akt (F,I). n = 3. Data are mean ± standard error of the mean. *P = .05, **P = .01, ***P = .001, 1-way or 2-way ANOVA.