A low-iron environment promotes the Mk bias observed in Tmprss6^−/− mice. (A) Schematic of color-coded BM transplantation groups. (B-D) Reconstituted peripheral blood analysis of recipients at indicated times after transplant. Week 0 represents nontransplanted mice. (E) Serum iron concentration of recipients 12 weeks posttransplant. (F) Surface expression of CD71 (fluorescence intensity) on MEPs harvested from transplant recipients. (G) Average number of colony types per 90 sorted MEPs harvested from transplant recipients. Data are presented as mean + SD. *P < .05, **P < .01, ***P < .001, and ****P = .0001 calculated by 2-tailed unpaired Student t test. KO, knockout.