TFR2 knockdown promotes Mk commitment of human MEP. (A-B) Knockdown efficiency of TFR2 assessed by mRNA (A) and protein (B) in human MEPs 48 hours after transduction (n = 3). (C) Proliferation index of carboxyfluorescein succinimidyl ester–stained MEPs transduced with 2 different TFR2-directed shRNAs after 72 hours in culture (n = 4 per group). Colony counts by type per 100 transduced MEPs per group (D), and average frequency of colony types (n = 4) (E). Data are presented as mean + SD. *P < .05, **P ≤ .01 calculated by 2-tailed unpaired Student t test compared to Scramble control.