PN-1 deficiency limits tail bleeding in mice with an F8 deficiency. Tail bleeding times and volumes in WT, PN-1^−/−, F8^−/− and F8^−/−/PN-1^−/− mice in a classical tail clip model (A-B), in a tail vein transection model (C-D), and in a tail artery transection model (E-F). Each dot represents 1 mouse. A nonparametric 1-way ANOVA test followed by a multiple comparison test was used. *P < .05; **P < .01; ***P < .001.