PN-1 deficiency effect in mice with an F8 deficiency during the mesenteric vessel ferric chloride-induced injury model. Quantitative analysis of platelet accumulation and fibrin generation after deposit of FeCl₃ to mesenteric vessels. Mice were injected with Alexa 647-labeled antibodies against glycoprotein IX and with AF488-labeled fibrinogen to monitor platelet accumulation and fibrin generation respectively. Representative images obtained 30 min after the start of recording (A). Platelet (B,D) and fibrin accumulation (C,E) in arterioles (B-C) and in venules (D-E). The mean of fluorescence intensity ± SEM is shown (n = 10-12). A 2-way ANOVA test followed by a multiple comparison test was used. *P < .05; **P < .01; ***P < .001; ****P < .0001.