Figure 5.
α1-Antitrypsin variants SMTR/V and SLLR/V are powerful inhibitors of bradykinin formation. (A) Inhibition of kaolin-induced contact system enzyme activity by SERPINs in C1INH-inactivated plasma (by 3.37 μM VAG8), measured with a chromogenic substrate. The dotted line indicates the level of inhibition that is achieved by plasma C1INH (ie, in absence of VAG8); 100% indicates full inhibition of enzyme activity. (B) Inhibition of kaolin-induced contact system enzyme activity by SERPINs in normal pooled plasma (with normal C1INH activity), measured with a chromogenic substrate; 100% indicates full inhibition of enzyme activity. (C) Kaolin-induced HK consumption after 15 minutes (quantification of repeated experiments in panel D). (E) Bradykinin formation after 5 minutes in the presence of a concentration range of SERPINs. Data represent the mean ± SD of 3 separate experiments, each performed in duplicate. §P < .0001, compared with pdC1INH by 1-way ANOVA.

α1-Antitrypsin variants SMTR/V and SLLR/V are powerful inhibitors of bradykinin formation. (A) Inhibition of kaolin-induced contact system enzyme activity by SERPINs in C1INH-inactivated plasma (by 3.37 μM VAG8), measured with a chromogenic substrate. The dotted line indicates the level of inhibition that is achieved by plasma C1INH (ie, in absence of VAG8); 100% indicates full inhibition of enzyme activity. (B) Inhibition of kaolin-induced contact system enzyme activity by SERPINs in normal pooled plasma (with normal C1INH activity), measured with a chromogenic substrate; 100% indicates full inhibition of enzyme activity. (C) Kaolin-induced HK consumption after 15 minutes (quantification of repeated experiments in panel D). (E) Bradykinin formation after 5 minutes in the presence of a concentration range of SERPINs. Data represent the mean ± SD of 3 separate experiments, each performed in duplicate. §P < .0001, compared with pdC1INH by 1-way ANOVA.

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