Identification of WFA⁺-M2BP–positive cells in immunofluorescent images. (A) Representative immunofluorescent images of WFA (green), M2BP (red), and DAPI (blue). Liver (upper panels) and lung (lower panels) sections from Patients 1, 2, and 3 are shown. WFA⁺-M2BP–positive cells in liver sections from Patients 1 and 2. Arrowheads point to examples of WFA⁺-M2BP–positive cells. Scale bars, 50 μm. (B) Quantification of WFA⁺-M2BP–positive cells in liver sections. The total number of WFA⁺-M2BP–positive cells was counted in 20 fields of view for each patient at original magnification ×200. Data are shown as box-and-whisker plots that indicate the 90th and 10th percentiles. Horizontal black lines represent median values, and circles indicate outliers. The Kruskal-Wallis nonparametric comparison with the Bonferroni adjustment was used for multiple comparisons. (C-D) Higher-magnification views of the area outlined by the dashed box in panel A. Scale bars, 50 μm. Representative immunofluorescent images of WFA (green), M2BP (red), and DAPI (blue) (C) and WFA (green), CD68 (red), and DAPI (blue) (D).