Effect of increasing FV concentrations on thrombin generation in 5 F5F8D plasmas. F5F8D plasma was titrated with increasing concentrations of FV (baseline FV, 25 IU/dL, 50 IU/dL, or 100 IU/dL), and PH (A) and LT (B) values of thrombin generation were plotted as a function of FV in the absence of anti-TFPI antibody. PH values were expressed as percentages of the maximal PH (PH %). (C) Effect of increasing FV concentrations on PH values in the presence of anti-TFPI antibody. (D) Comparison of average PH values in the absence and presence of the anti-TFPI antibody. Average PH values are presented as mean ± standard deviation.