Hippo kinase deletion cooperates with JAK2-V617F to activate innate immune response and proinflammatory cytokine production. (A) Experimental schematic for array-based serum cytokine profiling in JAK2-V617F MPN model in vivo. (B) Heatmap displaying the relative abundance of the most uniquely enriched (top) or depleted (bottom) cytokines in V617F-Stk4^+/−Stk3^+/− mice (based on mean z scores of 3 independent mice). (C) Venn diagram comparing the significantly more abundant (false discovery rate < 0.1) cytokines in either V617F-Stk4^+/+Stk3^+/+ group (left) or V617F-Stk4^+/−Stk3^+/− group (right) when compared against 6 vector-transduced mice. (D) Table depicting the results of Ingenuity Pathway Analysis of top predicted upstream regulators, based on submission of a ranked list of relative cytokine abundance z scores in V617F-Stk4^+/−Stk3^+/− against a reference background of the entire set of measured cytokines in the array. Upstream regulator name, predicted activation state (activated or inhibited), and adjusted P-values are indicated.