The combination of INCB053914 and ruxolitinib augments the suppression of biomarker phosphorylation indicative of mTORC signaling. Cell lysates of SET2 cells (A) and BaF3-JAK2^VF cells (B) incubated with INCB053914 and ruxolitinib alone and in combination as indicated for 4 hours (A) or 8 hours (B) were immunoblotted to assess the effects on levels of the indicated proteins. The white and black arrows indicate the migration of hyperphosphorylated and hypophosphorylated 4EBP1 protein, respectively. Note that the vinculin blot in panel A and Figure 3B are the same because these were the same drug-treated cells and subsequent lysates analyzed in each figure.