Figure 3.
Overexpression of TAZ induces cell death. (A) Proliferation assay of KMM1 cells transduced with empty vector (WPI), wild-type TAZ (TAZ), or TAZ mutants unable to bind TEAD (TAZ-F52A/F53A) or LATS1/2 (TAZ-WWm). Data are mean ± standard deviation (SD) of triplicates. Single-factor analysis of variance (ANOVA) of doubling times indicated significance between all TAZ-expressing lines and either KMM1 or WPI cell lines. (B) Cell-viability assay of cell lines described in panel A after 3, 6, or 9 days posttransduction. Data are mean ± SD of triplicates. *P ≤ .05 of TAZ, #P ≤ .05 of TAZ-F52A/F53A, or $P ≤ .05 of TAZ-WWm compared with KMM1 or WPI cell lines, respectively, using a Student t test. (C) Immunoblot of cell lines described in panel A for markers of apoptosis including cleaved caspase 3 and the 3 isoforms of BIM (BIMEL, BIML, BIMS). β-actin was used as a loading control.

Overexpression of TAZ induces cell death. (A) Proliferation assay of KMM1 cells transduced with empty vector (WPI), wild-type TAZ (TAZ), or TAZ mutants unable to bind TEAD (TAZ-F52A/F53A) or LATS1/2 (TAZ-WWm). Data are mean ± standard deviation (SD) of triplicates. Single-factor analysis of variance (ANOVA) of doubling times indicated significance between all TAZ-expressing lines and either KMM1 or WPI cell lines. (B) Cell-viability assay of cell lines described in panel A after 3, 6, or 9 days posttransduction. Data are mean ± SD of triplicates. *P ≤ .05 of TAZ, #P ≤ .05 of TAZ-F52A/F53A, or $P ≤ .05 of TAZ-WWm compared with KMM1 or WPI cell lines, respectively, using a Student t test. (C) Immunoblot of cell lines described in panel A for markers of apoptosis including cleaved caspase 3 and the 3 isoforms of BIM (BIMEL, BIML, BIMS). β-actin was used as a loading control.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal