Sptlc1 deletion affects myeloid progenitors and increases ST-HSC and MPP populations in BMT cells. (A) The LSK and LK populations of HSPCs from transplanted BM were analyzed by Sca-1 and c-Kit staining 21 days after poly(I:C) injection. (B) The total number of LSK cells was plotted for the Sptlc1^+/+ and Sptlc1^−/− mice (n = 11). (C-E) The total numbers of LT-HSCs, ST-HSCs, and MPPs were plotted for the Sptlc1^+/+ and Sptlc1^−/− mice (n = 11). (F-H) The total numbers of CD150⁺ CD48⁻, CD150⁻CD48⁻, and CD150⁻CD48⁺ were plotted for the Sptlc1^+/+ and Sptlc1^−/− mice (n = 11). (I-K) The total numbers of MPP2, MPP3, and MPP4 were plotted for the Sptlc1^+/+ and Sptlc1^−/− mice (n = 11). (L) The total number of LK cells was plotted for the Sptlc1^+/+ and Sptlc1^−/− mice (n = 11). (M-O) The total numbers of CMPs, GMPs, and MEPs were plotted for the Sptlc1^+/+ and Sptlc1^−/− mice (n = 11). All graphs data are represented as mean ± SEM. P < .05 is significant, calculated from the unpaired Student t test.