Figure 2.
HBA cell subset-specific expression of chemokines at E10.5. (A) Flow cytometric sorting strategy for enrichment of MCs (CD31−CD41−CD45−), ECs (CD31+CD41−CD45−), and HCs (CD41+ and/or CD45+). (B) Relative expression of messenger RNA (mRNA) for chemokine genes Cx3cl1, Cxcl12, Ccl3, and Ccl11 as normalized to actin in E10.5 HBA sorted MC, EC, and HC. Standard error of the mean (SEM) is shown. n = 5; *P < .05; **P < .01.