Cdx4 promotes expression of genes involved in oncogenic pathways and suppresses genes associated with erythroid differentiation. (A) Volcano plot showing all genes in the RNA sequencing analysis >P < .05. Each green dot represents a gene that is not differentially regulated. Upregulated DEGs in HSPCs, fold change >1.5 are indicated in red, downregulated DEGs in blue. Cumulative data of 3 biological independent experiments are shown for the vector control and Cdx4. (B) Heat map showing selected DEGs determined by RNA-Seq of Cdx4 and vector control-transduced HSPCs. Heat map representing unsupervised hierarchical clustering of all 6 samples for DEGs (fold change >1.5; P < .05). (C) Enrichr, ChEA, and ENCODE databases consensus transcription factor (TFs) enrichment analysis of downregulated (DN) DEGs, demonstrating enrichment for known targets of Gata1 and Gata2. (D) ENCODE and ChEA consensus TFs from ChIP-X analysis of downregulated DEGs (upper) and upregulated DEGs (lower). (E) The Molecular Signatures Database (MSigDB) oncogenic signature analysis of Cdx4 induced DEGs. (F) Kyoto Encyclopedia of Genes and Genomes (KEGG) 2019 pathway analysis of Cdx4 induced DEGs. The analysis in panels D-F was performed on Enrichr, web-based analysis platform.⁴⁰