Genes upregulated in the RNA-Seq analysis of Cdx4 transduced murine HSPCs show an enrichment of AML LSC signature genes and overlap with protein coding genes in human AEL. (A) GSEA analysis based on RNA-Seq of Cdx4 transduced HSPCs, showing enrichment of human LSC signature.²⁷  Right, upregulated genes significantly enriched for the human LSC signature are shown as heat map. (B) Venn diagram representing the overlap of the DEGs on Cdx4 overexpression (P < .05) with published RNA sequencing data (protein coding genes) in human patients with AEL.⁵   NES, normalized enrichment score.