TF and PAI-1 levels in the endothelium of IVC of HT-29–injected mice are suppressed by an AHR antagonist. IVCs of mice were harvested and divided for immunoblotting (as shown in Figure 7E-F) and fixed for immunofluorescence microscopy. (A,C) Immunofluorescence images of IVC stained with anti-TF and anti–PAI-1 along with anti-CD31. The secondary antibodies consisted of Alexa Fluor. Green = CD31; red = TF or PAI-1. 4′,6-Diamidino-2-phenylindole (DAPI) was used to stain the nuclei. Representative images of 5 IVC mice are shown. Scale bar = 10 μm for TF and 30 μm for PAI-1. (B,D). Three images per IVC were analyzed, and a region of interest was marked corresponding to the endothelial cells. Integrated density was normalized to the surface area of the region of interest measured by using ImageJ as microns. The data are represented as box plots, and a Student t test was used to compare both the groups, **P = .005 for TF and P < .001 for PAI-1.