HOXA10-AS is a prognostic factor in AML. Analysis of RNA-sequencing data from 258 NCI-TARGET pediatric³⁵  (A-B) and 171 TCGA adult³⁶  (C-D) AML samples. (A) t-SNE representation using the 4362 most variable genes (interquartile range >1.5), with samples colored according to AML subtype. (B) t-SNE as in panel A with samples colored according to HOXA10-AS expression as indicated by the color key. (C) t-SNE representation using the 1840 most variable genes (interquartile range >1.5), with samples colored according to AML subtype. (D) t-SNE as in panel C with samples colored according to HOXA10-AS expression as indicated by the color key. (E) Probability of EFS in 258 NCI-TARGET pediatric AML patients with high (red; >5.5 normalized reads; cutoff determined via maximally selected rank statistics) or low HOXA10-AS expression (blue; ≤5.5 normalized reads). (F) Probability of EFS in 171 TCGA adult AML patients with high (red; >4.6 normalized reads; cutoff determined via maximally selected rank statistics) or low HOXA10-AS expression (blue; ≤4.6 normalized reads).