Cellular kinetics of CAR19 and CAR22 transgenes in peripheral blood. Copies of CAR19 (A) and CAR22 (B) transgenes, respectively, in patients with B-ALL and copies of CAR19 (C) and CAR22 (D) transgenes in patients with B-NHL. Genomic DNA was isolated from the samples of whole blood at serial time points before and after infusion of the CAR T-cell cocktail. Each panel represents the cellular kinetics of CAR transgenes within the first 18 months since infusion. The red horizontal line in each panel denotes the lower limit of quantitation (50 copies/μg).