Donor T-cells lacking intact Itpkb activity can mediate a GVL effect against A20^luc lymphoma. (A) Survival, weight, and BLI results of lethally irradiated BALB/c recipients infused with B6 BM, with or without A20^luc lymphoma cells (0.75 × 10⁶), with or without supplemental B6 Itpkb^+/+ or Itpkb^−/− purified T cells (2 M is 2 × 10⁶ cells). (B) Survival, weight and BLI results of lethally irradiated BALB/c recipients infused with B6 BM, B6 WT purified T cells (2 × 10⁶), and A20^luc lymphoma cells (0.75 × 10⁶) on day 0, then treated with vehicle or GNF362 (days 0-42). (C) Tumor burden was quantified using BLI at the indicated time points after BMT (n = 5 mice/BM group; n = 5 mice/BM+A20^luc lymphoma group; n = 6-8 mice/BM+A20^luc lymphoma+T group). One experiment was performed. Data are shown as the mean ± SEM. *P < .05, **P < .01, ***P < .001, and ****P < .0001.