KIR2DL2/L3 single⁺NK cells in the d-rsKIR group (C1Cx-C1Cx) show higher reactivity compared with other groups (dsKIR and rsKIR). The percentage of KIR2DL2/L3 single⁺ NK cells at day 90 (A) and day 180 (B) after transplantation in the d-rsKIR group (pairs of donor C1Cx and host C1Cx, n = 96), dsKIR group (pairs of donor C1Cx and host C2C2, n = 7), and rsKIR group (pairs of donor C2C2 and host C1Cx, n = 10). Secretion of IFN-γ (C) of KIR2DL2/L3 single⁺ NK cells against K562 cells at day 90 after transplantation in the d-rsKIR, dsKIR, and rsKIR groups. MFI expression of DNAM-1 on KIR2DL2/L3 single⁺ NK cells at day 90 (D) and 180 (E) after transplantation in the d-rsKIR, dsKIR, and rsKIR groups. Heatmap of average NK-cell marker expression on single KIR2DL2/L3⁺ NK cells from each group at different time points. Sample origin color-coding according to the color key in panel F. In order to adjust all the data to the same order of magnitude, we process the data as follows: the MFI of DNAM-1 and Bcl-2 was divided by 100, the MFI of NKP30, NKP46, and CD122 was divided by 10, and the expression of CD25 was multiplied by 10.