Platelet-targeted FVIII expression restores functional hemostatic properties in Dahl-FVIII^−/−rats that received 2bF8^tg-BMT. Five months after receiving BMT from 2bF8^tg rats, blood samples were collected from Dahl-FVIII^−/− recipients via the ventral tail artery. Sodium citrate was used as an anticoagulant. Blood samples were recalcified for ROTEM analysis and TGA. Blood samples from Dahl-WT and Dahl-FVIII^−/− rats were used as controls. (A) Native ROTEM analysis of the whole-blood coagulation status in Dahl-FVIII^−/− recipients after receiving BMT from 2bF8^tg rats. The parameters analyzed included clotting time, clot formation time, A10 amplitude, and α-angle. (B) Evaluation by nWB-TGA of the hemostatic system in Dahl-FVIII^−/− recipients after receiving BMT from 2bF8^tg rats. The parameters analyzed include lag time, peak time, thrombin generation rate, and endogenous thrombin potential.