Protein expression of FXIII-A variants. (A) ELISA results showing the expression of the FXIII-A variants relative to the wild-type FXIII-A. Data are shown as the mean from 3 experiments with error bars representing standard deviations. (B) Western blot showing the FXIII-A variants detected with a monoclonal anti–FXIII-A antibody. Controls include a molecular weight marker (MW), lysate from untransfected CHO cells (UT) as negative control, lysate from CHO cells transfected with wild-type FXIII-A (WT), and commercially available rFXIII-A (Zedira) as positive control (20 ng and 50 ng loaded).