NFAT controls the expression of IL-6, IL-10, and c-Jun. (A) Heatmaps of differentially expressed genes in HBL-1 treated for 12, 24, 36, and 48 hours with CsA compared with the solvent control. Gene expression changes are depicted according to the color scale. (B,C) HBL-1 cells were treated with solvent, CsA, or FK506; mRNA levels of the indicated genes were quantified by qPCR analysis. (D) JUN and IL10 mRNA expression were measured by qPCR in the indicated ABC DLBCL cell lines. (E) IL-6 and IL-10 protein levels were quantified in the medium of solvent- or CsA-treated ABC DLBCL cell lines by ELISA and normalized to the DMSO control. (F) The indicated ABC DLBCL cell lines were treated for 48 hours with solvent, CsA or FK506 and cell lysates were analyzed by western blotting. (G) TMD8 cells were treated with solvent or the calcineurin inhibitors for 48 hours and part of the cells were incubated with PMA for 6 hours subsequently. Adhesion to BSA- or fibronectin-coated plates was measured by an MTS assay. Adhesion was normalized to solvent-treated cells bound to BSA-coated plates. (A-G) Data are representative of at least 3 independent experiments. Error bars correspond to the mean ± SD. Statistical significance was calculated using Student t test (*P < .05, **P < .01, ***P < .001). PMA, phorbol 12-myristate 13-acetate.