Combinatorial treatment with CFZ and AGI-6780 causes a reduction in IDH2 activity and mATP levels. (A) KMS-27 and (B) KMM-1^PIR cells UT, treated with DMSO, CFZ (2.5 nM and 5 nM, respectively), AGI-6780 (5 µM), or a combination of the 2 drugs were analyzed for IDH2 activity 6 hours posttreatment. (C) KMS-27 and (D) KMM-1^PIR cells treated as described previously were analyzed for TCA cycle activity 6 hours posttreatment. Data are the means ± SD of 4 independent experiments. (E) KMS-27 cells treated as described previously were analyzed for ETC complexes I to III 7 hours posttreatment. (F) KMS-27 cells treated as described previously were analyzed for mATP production 7 hours posttreatment. Data are the means ± SD of 3 independent experiments (*P < .05; **P < .01; ***P < .001). mATP, mitochondrial ATP.