Effects of α-Def-1 on fibrin structure. (A) Representative 3D confocal microscopy images of fibrin clots formed from fibrinogen in the absence (left) or presence (right) of 5 µM α-Def-1. Fibrin was visualized using Alexa Fluor 647–labeled fibrinogen. (B) Representative scanning electron micrographs of fibrin clots formed in the absence (left) or presence (right) of 5 μM α-Def-1 at original magnification ×2000 and ×10 000. Three individual clots were studied at each experimental condition. Scale bars represent 30 μm (top) and 5 μm (bottom).