Rag deficiency prevents MPD.Rag^KO and Rag^KO/CD11c^ΔMHCII mice were assessed by flow cytometry at 1 year of age. WT and CD11c^ΔMHCII mice are shown for comparative purposes. (A) Spleen weight (n = 23, 25, 8, 9). Dashed line indicates the threshold for splenomegaly (2 standard deviations above the mean of WT mice from all age groups). (B) Spleen weight/total weight ratio (n = 22, 25, 8, 9). (C) Absolute number of peripheral mononuclear neutrophils (CD11b^highGr1⁺) among total splenocytes. (n = 22, 23, 7, 8). Dashed line indicates the threshold for neutrophilia (2 standard deviations above the mean of WT mice from all age groups). (D) Absolute number of neutrophil precursor-like cells among total splenocytes (n = 22, 23, 7, 8). Dashed line indicates threshold for higher neutrophil precursors (2 standard deviations above the mean of WT mice from all age groups). (E) Ratio of granulocytes to erythroid cell precursors in the bone marrow (BM) (n = 10, 17, 8, 9). Dashed line indicates threshold for higher ratio (2 standard deviations above the mean of WT mice from all age groups). Mean ± standard error of the mean and individual data points are shown.