Efficient hepatocyte-specific ablation of Tfr1 in Tfrc^Alb-Cremice. Livers from 8-week-old female and male Tfrc^Alb-Cre mice and control Tfrc^fl/fl littermates (n = 10 for each group) were used for biochemical and histological assessment of Tfr1 expression. (A) qPCR analysis of Tfr1 mRNA. Data are mean ± standard error of the mean. (B) Western blot analysis of Tfr1. (C) Immunohistochemical detection of Tfr1; arrows denote Tfr1 expression in nonparenchymal liver cells. Scale bars, 200 μm (upper panels), 100 μm (lower panels); original magnification ×10 and ×20. (D) Immunofluorescence detection of Tfr1 and the early endosome marker EEA1; colocalization is shown by the white arrowheads. Scale bar, 2 μm; original magnification ×63. Statistical analysis was done using 2-way ANOVA.