Tfrc^Alb-Cremice dysregulate hepcidin expression and develop mild hypoferremia and microcytosis. Eight-week-old female and male Tfrc^Alb-Cre mice and control Tfrc^fl/fl littermates (n = 10 for each group) were euthanized. Blood was isolated by cardiac puncture and used to analyze hemoglobin (A), red blood cell count (B), and MCV (C). Sera were prepared for analysis of iron (D), TIBC (E), transferrin saturation (F), ferritin (G), and hepcidin (J). Livers were dissected and used to analyze LIC (H) and Hamp mRNA (I). Values for Hamp mRNA, serum hepcidin, and LIC were used to calculate Hamp/LIC (K) and hepcidin/LIC (L) ratios. All data are mean ± standard error of the mean. Statistically significant differences across genotypes are indicated by P values (or ns [nonsignificant]) and across sexes by ***P < .001, 2-way ANOVA.