Tfrc^Alb-Cremice are predisposed to iron-deficiency anemia. Twelve-week-old male Tfrc^Alb-Cre mice and control Tfrc^fl/fl littermates (n = 4-6 for each genotype) were placed on an IDD for 12 weeks. At the end point, the animals were euthanized. Blood was isolated by cardiac puncture and used for hematological analysis. Serum was prepared, and livers were dissected to analyze iron parameters. (A) Serum iron. (B) TIBC. (C) Transferrin saturation. (D) Serum ferritin. (E) LIC. (F) Liver Hamp mRNA. (G) Liver Hamp/LIC ratios. (H) Liver Tfr1 mRNA. (I) Hemoglobin. (J) Hematocrit. (K) Red blood cell count. (L) MCV. Data are mean ± standard error of the mean. Dashed lines indicate average values obtained from age-matched male C57BL/6 mice on an SD (n = 3). Statistical analysis was performed using the Student t test.