Tfrc^Alb-Cremice respond appropriately to dietary iron challenges. Eight-week-old male Tfrc^Alb-Cre mice and control Tfrc^fl/fl littermates (n = 30 for each genotype; n = 5 for each treatment) were placed on an SD or an IDD for 5 days or an HID for 7 days. On the last day of the IDD, mice remained on this diet or were switched to an HID for 3 or 6 hours. On the last day of the HID, mice remained on this diet or were switched to an IDD for 24 hours. Sera were prepared for the analysis of iron (A), TIBC (B), transferrin saturation (C), and ferritin (D). Livers were dissected and used to analyze LIC (E), Hamp mRNA (F), Hamp/LIC ratios (G), Tfr1 mRNA (H), and Bmp6 mRNA (I). (J) Liver lysates were used for western blot analysis of Tfr1, Tfr2, ferritin, and β-actin. All data are mean ± standard error of the mean. Statistical analysis was performed using multiple t tests.