CCND1 affects histone modifications at the SOX11 locus. (A) ENCODE H3K9Ac ChIP sequencing (seq) data for H1-hESC cells show SOX11 gene regions that have positive ChIP peak signals. Arrows indicate regions where PCR primers were designed. (B-F) H3K9/14Ac chromatin immunoprecipitation assays for the SOX11 gene from indicated cells stably transduced with empty vector or CCND1 (B-E) or treated with 1 μM of SAHA for 16 hours (F). Bar graphs show means of qPCR signals of DNA region 1 or 2 (amplicons 1 and 2) pulled down by the H3K9/14Ac antibody as fold enrichment relative to the background signals from the isotype control immunoglobulin G (IgG) antibody. Error bars, standard deviation. (G) Immunoblot analysis of Z-138 cells treated with 2 μM of SAHA for 3 h and immunoblotted with indicated antibodies. ***P < .001, ****P < .0001 by 2-sided Student t test. DMSO, dimethyl sulfoxide; UTR, untranslated region.