CCND1 interacts with HDAC1 and HDAC2. (A) HEK-293T cells were transiently cotransfected with untagged CCND1 and individual HA-tagged HDACs and immunoprecipitated (IP) with HA antibody followed by immunoblotting (IB) with indicated antibodies. Lysates before immunoprecipitation were used as input samples. Arrow indicates specific bands for HDAC4-HA. (B) Z-138 cells or primary MCL samples were immunoprecipitated with isotype control immunoglobulin G (IgG), CCND1, or HDAC1 antibody and immunoblotted with indicated antibodies. Lysates before immunoprecipitation were used as input samples. Arrow indicates specific HDAC1 staining. (C-D) Z-138 and JEKO-1 cells were stably transduced with control, HDAC1 (C), or HDAC2 (D) shRNA, and protein expression was analyzed by immunoblot analysis with indicated antibodies 3 days after transduction. *Nonspecific (n.s.) bands.