American Society of Hematology

Figure 4.

$Figure 4. Reduced chromatin localization of HDAC1 in CCND1 overexpressing cells. (A) Chromatin immunoprecipitation assays for the SOX11 gene from Z-138 cells stably transduced with empty vector (EV), WT, or mutant Y44D CCND1. Bar graphs show means of quantitative PCR signals of region 1 (amplicon 1) pulled down by the HDAC1 antibody as fold enrichment relative to the background signals from the isotype control immunoglobulin G (IgG) antibody. Error bars, standard deviation. (B) Cytosolic, soluble, and insoluble nuclear extracts were prepared as described in “Materials and methods” from Z-138 cells that stably expressed empty vector or WT CCND1-HA. The extracts were immunoblotted with indicated antibodies. LAMIN A/C and histone H3 were used to confirm nuclear fractions. ****P < .0001 by 2-sided Student t test. cyto, cytoplasmic; insol, insoluble nuclear fraction; Nucl, soluble nuclear fraction.$

Reduced chromatin localization of HDAC1 in CCND1 overexpressing cells. (A) Chromatin immunoprecipitation assays for the SOX11 gene from Z-138 cells stably transduced with empty vector (EV), WT, or mutant Y44D CCND1. Bar graphs show means of quantitative PCR signals of region 1 (amplicon 1) pulled down by the HDAC1 antibody as fold enrichment relative to the background signals from the isotype control immunoglobulin G (IgG) antibody. Error bars, standard deviation. (B) Cytosolic, soluble, and insoluble nuclear extracts were prepared as described in “Materials and methods” from Z-138 cells that stably expressed empty vector or WT CCND1-HA. The extracts were immunoblotted with indicated antibodies. LAMIN A/C and histone H3 were used to confirm nuclear fractions. ****P < .0001 by 2-sided Student t test. cyto, cytoplasmic; insol, insoluble nuclear fraction; Nucl, soluble nuclear fraction.

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