STAT3 negatively regulates SOX11 expression. (A) JEKO-1 and JVM-2 cells were transduced with empty vector (EV) or CCND1-HA, and cell lysates were immunoblotted with indicated antibodies. (B) Box plots of relative SOX11 and CCND1 mRNA expression in primary MCL cases. Gene expression data for SOX11 and CCND1 were obtained from GSE16455³⁴  (supplemental Table 6) and plotted using GraphPad Prism v7.0a. (C-D) Immunoblot analysis of MCL cell lines (C) or MCL PDXs and primary MCL cases (D) with indicated antibodies. (E) Indicated MCL cell lines were treated with indicated doses of the JAK1/2 inhibitor AZD1480 for 16 hours and immunoblotted with indicated antibodies. (F) Indicated MCL cell lines were treated with AZD1480 as in panel E, and SOX11 mRNA was analyzed by qPCR. Shown are the means of mRNA expression levels after normalization to GAPDH signals from 4 independent amplification experiments. (G) JEKO-1 or GRANTA-519 cells were transduced with control or STAT3 shRNA, and protein lysates were prepared for immunoblot analysis with indicated antibodies. (H) Indicated MCL lines were treated with 500 nM of AZD1480 for 16 hours, and SOX11 mRNA was analyzed by qPCR. Bar graphs show the means of mRNA expression levels after normalization to GAPDH signals from 4 independent amplification experiments. Error bars, standard deviation. **P < .01, ***P < .001, ****P < .0001 by 2-sided Student t test. n.s. nonsignificance.