American Society of Hematology

Figure 6.

$Figure 6. Effects of IL-21 on STAT3 activity, SOX11 expression, and cell viability in MCL cells. (A) Indicated MCL cell lines were treated with 50 ng/mL of IL-21 for 96 hours, and SOX11 mRNA was analyzed by qPCR. Shown are the means of mRNA expression levels after normalization to GAPDH signals from 4 independent amplification experiments. (B) Immunoblot analysis of indicated MCL cell lines treated as described in panel A. (C) Immunoblot analysis of MCL PDX models treated with 50 ng/mL of IL-21 for 72 hours. (D) Indicated MCL cell lines were treated with 50 ng/mL of IL-21, and viable cells (propidium iodide (PI) negative) were assessed by flow cytometry at indicated times. Shown are the means of PI− fractions compared with untreated samples from at least 2 independent experiments. (E) MCL PDX cells were treated with IL-21, and viable cells were analyzed as in panel D for the indicated times. Shown are the means of PI− fractions compared with untreated samples from at least 2 independent experiments. Error bars, standard deviation. **P < .01, ***P < .001, ****P < .0001 by 2-sided Student t test.$

Effects of IL-21 on STAT3 activity, SOX11 expression, and cell viability in MCL cells. (A) Indicated MCL cell lines were treated with 50 ng/mL of IL-21 for 96 hours, and SOX11 mRNA was analyzed by qPCR. Shown are the means of mRNA expression levels after normalization to GAPDH signals from 4 independent amplification experiments. (B) Immunoblot analysis of indicated MCL cell lines treated as described in panel A. (C) Immunoblot analysis of MCL PDX models treated with 50 ng/mL of IL-21 for 72 hours. (D) Indicated MCL cell lines were treated with 50 ng/mL of IL-21, and viable cells (propidium iodide (PI) negative) were assessed by flow cytometry at indicated times. Shown are the means of PI⁻ fractions compared with untreated samples from at least 2 independent experiments. (E) MCL PDX cells were treated with IL-21, and viable cells were analyzed as in panel D for the indicated times. Shown are the means of PI⁻ fractions compared with untreated samples from at least 2 independent experiments. Error bars, standard deviation. **P < .01, ***P < .001, ****P < .0001 by 2-sided Student t test.

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