pY705 STAT3 is directly recruited to the SOX11 gene. (A) Diagram of the SOX11 gene and enhancer (not drawn to scale; top). Arrows indicate STAT3 binding sites. JEKO-1 cells were treated with 500 nM of AZD1480 or dimethyl sulfoxide (DMSO) for 16 hours, and chromatin immunoprecipitation assays were performed using the pY705 STAT3 antibody (bottom). (B) Chromatin immunoprecipitation assays using isotype immunoglobulin G (IgG) or H3K9/14Ac antibody for MAVER-1 cells treated with 500 nM of AZD1480 or DMSO for 16 hours. (A-B) Bar graphs show means of qPCR signals from 4 independent amplification experiments using primers to regions 1 to 4 (amplicons). Data are shown as percentage of total input chromatin DNA. Error bars, standard deviation. (C) Proposed model of SOX11 expression through distinct mechanisms mediated by CCND1 and STAT3 in typical or indolent form of MCL. *P < .05, **P < .01, ***P < .001, ****P < .0001 by 2-sided Student t test. TF, transcription factor.