Deficiency of Wdr26 leads to enlarged nuclei with elevated protein abundance in differentiating erythroblasts. (A) Giemsa and DAPI staining of wild-type and wdr26b^−/− zebrafish blood cells. Scale bars, 10 μm (Giemsa) and 5 μm (DAPI). (B) Quantification of nuclear size of zebrafish blood cells in panel A. Three pairs of wild-type and wdr26b^−/− fish were analyzed. At least 100 cells were quantified for each genotype. **P < .01. (C) DAPI staining showed enlarged nuclei in Wdr26-silencing mouse primary erythroblasts. Scale bars, 20 μm. DAPI, 4′,6-diamidino-2-phenylindole; MPFE, mouse primary fetal liver erythroblast. (D) Quantitative analysis of the nuclear size of control and Wdr26-silencing mouse primary erythroblasts. More than 50 cells are shown for each shRNA. (E) DAPI staining showed enlarged nuclei in chemically induced Wdr26-knockout MEL cells. Scale bars, 10 μm. (F) Quantification of nuclear size in panel E. At least 100 cells were quantified for each clone. (G) Western analysis of nuclear proteins in DMSO-induced wild-type and Wdr26-knockout MEL cells. (H) mRNA expression of genes encoding the nuclear proteins shown in panel G. Error bars represent SEM from 2 replicates in an RNA-seq experiment. *P < .05, **P < .01.