Silencing of hepatocyte Plat mRNA in WT mice decreases liver tPA, plasma tPA, and systemic fibrinolytic activity.Plat mRNA (A), tPA concentration by ELISA (B), and tPA activity by enzymatic assay (C) in the liver and carotid arterial lysates of WT mice injected with control AAV8-H1 virus or AAV8-H1-shPlat. Results are shown as mean ± SEM (n = 4 mice per each group). Plasma tPA protein concentration (D), plasma tPA activity (E), tail bleeding time (F), and time to occlusive carotid arterial thrombosis (G) induced by photochemical injury in WT mice injected with control AAV8-H1 virus or AAV8-H1-shPlat (n = 8-10 mice per each group). (H) Plasma tPA concentration 1 week before (basal) and 20 minutes after FeCl₃-induced carotid artery thrombosis; tPA release was calculated by subtracting the basal value from the after-FeCl₃ value for each mouse. Horizontal lines in dot-density plots indicate mean values. *P < .05, **P < .01, ***P < .001, 2-tailed Student t test (A-C,E-H), Mann-Whitney U test (D). n.s., not significant (P ≥ .05).