Relationships among DACH1, ATF6, and PLAT in human liver. (A) Immunoblot of DACH1, with β-actin as a loading control, in lysates of liver specimens from 25 human subjects (supplemental Table 1). (B) ATF6 or PLAT mRNA was normalized to RPLP0. Liver DACH1:β-actin was quantified by densitometric analysis of the immunoblots in (A). Data were analyzed by the Fisher exact 2-tail tests for DACH1 <4 or >4 vs ATF6 <4 or >4 and vs PLAT <15 or >15. Statistically significant inverse associations were found for the gray areas in each graph, with the P values indicated. (C) Correlation of liver ATF6 and PLAT mRNA. The data were analyzed by linear regression, with the r² and P values indicated.