Figure 3. Marrow fibrotic tissue is... | American Society of Hematology

Figure 3.

$Figure 3. Marrow fibrotic tissue is composed of 2 distinct populations with identical morphology. (A-B) Femur sections of VDR+/+ and VDR−/− recipient mice transplanted with VDR+/+GFP+CD45+lineage−c-kit+ BM cells (2 months after transplantation): (A) H&E and silver staining and (B) GFP fluorescence. Scale bars, 500 μm (black) and 50 μm (white). (C-D) Immunohistochemical staining of F4/80, osterix, and α-SMA (C) and GFP expression and immunofluorescence staining of osterix (D) in marrow fibrotic tissue of VDR−/− recipient. Scale bars, 20 μm (black) and 20 μm (white). (E) VDR mRNA expression in lineage-positive (Lin+), Lin−c-kit+Sca1−, and Lin−c-kit+Sca1+ fractions from VDR+/+ BM (n = 3). Representative pictures or combined data of at least 3 independent experiments are shown. Data are represented as mean plus or minus SEM. **P < .01, ***P < .001 (ANOVA).$

Marrow fibrotic tissue is composed of 2 distinct populations with identical morphology. (A-B) Femur sections of VDR^+/+ and VDR^−/− recipient mice transplanted with VDR^+/+GFP⁺CD45⁺lineage⁻c-kit⁺ BM cells (2 months after transplantation): (A) H&E and silver staining and (B) GFP fluorescence. Scale bars, 500 μm (black) and 50 μm (white). (C-D) Immunohistochemical staining of F4/80, osterix, and α-SMA (C) and GFP expression and immunofluorescence staining of osterix (D) in marrow fibrotic tissue of VDR^−/− recipient. Scale bars, 20 μm (black) and 20 μm (white). (E) VDR mRNA expression in lineage-positive (Lin⁺), Lin⁻c-kit⁺Sca1⁻, and Lin⁻c-kit⁺Sca1⁺ fractions from VDR^+/+ BM (n = 3). Representative pictures or combined data of at least 3 independent experiments are shown. Data are represented as mean plus or minus SEM. **P < .01, ***P < .001 (ANOVA).

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